|
W - Weeks
|
Start date
|
End date
|
Antigen availability
|
|
|
Antigen selection and generation
|
Antigen concentration/fractionation
|
4
|
|
|
Antigen qualification/characterization
|
1D SDS-PAGE,
2D SDS-PAGE,
or Bradford assay
|
2
|
|
|
Immunization
|
Testing of pre-immune serum of each animal
|
4
|
|
|
Immunization
|
16
|
|
|
Testing of raw serum/purified antibody
|
4
|
|
|
Antibody Characterization
|
Purification of antisera
|
4
|
|
|
Antibody characterization
|
4
|
|
|
Preparation of reference standard
|
2
|
|
|
Assay Development and Testing
|
End point assay development
|
8
|
|
|
Assay Validation
|
8
|
|
|
Routine testing
|
4
|
|
|
Completed
|
|
|
|
Start date
|
End date
|